Technical Capabilities
Precision Instrumentation. Deep Analytical Expertise.
PuriSpec operates a fully equipped chromatographic and spectroscopic laboratory purpose-built for pharmaceutical and chemical research — from milligram-scale analytical work to multi-hundred gram preparative purification.
Supercritical Fluid Chromatography (SFC)
SFC is the cornerstone of PuriSpec's separation capabilities. Using supercritical CO₂ as the primary mobile phase — often modified with alcoholic co-solvents — SFC delivers faster separations, lower solvent consumption, and superior resolution for chiral and achiral compounds compared to conventional HPLC.
Speed
Typical SFC run times are 3–5× faster than equivalent HPLC methods, enabling rapid method development and high-throughput screening.
Green Chemistry
CO₂-based mobile phases dramatically reduce organic solvent waste — up to 80% less than HPLC — supporting sustainable laboratory practices.
Chiral Selectivity
SFC is uniquely suited to chiral stationary phases, providing exceptional enantioselectivity across a broad range of compound classes.
Scalability
Methods developed at analytical scale transfer directly to preparative scale, from milligrams to hundreds of grams, with minimal re-optimization.
Instrumentation
Analytical SFC/MS System
Coupled to single-quadrupole MS for real-time fraction analysis and purity confirmation during method development.
Preparative SFC Platform
High-flow preparative system capable of processing gram to multi-hundred gram quantities per run with stacked injection capability.
Chiral Column Library
20+ chiral stationary phases (Chiralpak, Chiralcel, Lux, RegisPack series) for comprehensive screening across diverse compound classes.
Chiral Separations
Stereochemistry is critical in drug development — enantiomers can differ dramatically in potency, selectivity, and toxicity. PuriSpec specializes in the resolution of racemic mixtures and the determination of enantiomeric excess (ee) for pharmaceutical intermediates and APIs.
Separation Capabilities
- Analytical chiral method development across SFC and HPLC platforms
- Enantiomeric excess (ee) determination by chiral HPLC and SFC
- Preparative chiral separation from milligrams to hundreds of grams
- Absolute configuration assignment via optical rotation and circular dichroism
- Chiral building block preparation for asymmetric synthesis programs
- Diastereomeric separation for complex multi-stereocentre compounds
Compound Classes
- Amino acids and peptide intermediates
- Pharmaceutical APIs and advanced intermediates
- Natural product-derived scaffolds
- Agrochemical and fine chemical intermediates
- Kinase inhibitors and oncology compounds
- CNS-active compounds and neuropharmaceuticals
API Purification
Delivering pharmaceutical-grade material requires more than separation — it demands rigorous purity assessment, impurity profiling, and full analytical documentation. PuriSpec's purification workflows are designed to meet the demands of IND-enabling studies and regulatory submissions.
| Scale | Quantity Range | Typical Application |
|---|---|---|
| Analytical | < 10 mg | Method development, ee determination, structural confirmation |
| Semi-preparative | 10 mg – 1 g | Early-stage biological evaluation, SAR studies |
| Preparative | 1 g – 50 g | Lead optimization, in vivo studies, IND-enabling batches |
| Process | 50 g – 500 g+ | Scale-up, CMC development, technology transfer |
Typical purity targets of ≥95% by HPLC-UV, with ≥99% achievable for regulatory-grade material. Full impurity profiling and residual solvent analysis available on request.
Analytical & Spectroscopic Methods
Structural confirmation and purity assessment are integral to every project. PuriSpec provides comprehensive analytical characterization packages to support regulatory submissions, partner review, and internal quality control.
HPLC-UV/ELSD
Reversed-phase and normal-phase HPLC for purity profiling, impurity identification, and quantitative analysis.
SFC-UV/MS
Analytical SFC with UV and single-quadrupole MS detection for rapid purity and identity confirmation.
LC-MS (ESI/APCI)
Electrospray and APCI ionization for molecular weight confirmation and impurity characterization across a broad mass range.
High-Resolution MS (HRMS)
Exact mass determination for molecular formula confirmation; essential for regulatory submissions and patent filings.
1H / 13C NMR
Full structural elucidation including 1D and 2D experiments (COSY, HSQC, HMBC) for unambiguous structural assignment.
Optical Rotation & CD
Specific optical rotation measurement and circular dichroism for stereochemical assignment and ee confirmation.
Molecular Weight Range: Analytical capabilities span molecular weights from ~100 Da (small fragments) to ~1500 Da (peptide intermediates and macrocycles), covering the full range of small-molecule drug discovery.
Discuss Your Technical Requirements
Our team is ready to evaluate your compound, purity targets, and timeline — and recommend the optimal separation and characterization strategy.